Abstract: This article describes the application of Shimadzu SPD-M20A diode array detector in LC-6AD preparative liquid chromatography. Use Shim-pack PRC-ODS 20 mmID. × 250 mmL. 15μm chromatographic column and water-methanol as mobile phase, using gradient elution and level collection to separate and collect the mixed drug components Purity testing.
Diode array detector, also known as photodiode array detector or photodiode matrix detector, is expressed as PDA (photo-diode array), PDAD (photo-diodearray detector) or (Diode array detector, DAD) in the 1980s An optical multi-channel detector appeared. Using a diode array detector as the detector for preparing a liquid chromatograph can greatly improve the detection efficiency, and overcome the influence of the difference in response of different compounds in the sample due to different characteristic absorption wavelengths on the collection results.
The Shimadzu SPD-M20A has 512 photodiodes and a detection wavelength range of 190-800 nm. Its characteristic is that it can obtain ultraviolet absorption information at each wavelength within a set wavelength range with a single injection, and the data processing is faster, which can not only overcome The shortcomings of ordinary ultraviolet-visible absorption detectors, and can also obtain three-dimensional spectral chromatograms of chromatographic separation components, providing analysts with very rich qualitative and quantitative information. PDA data can be used to establish a sample preparation and separation method suitable for multiple absorption characteristics in a single injection purification. In addition, the peak purity and spectrum of the separated target peak can be obtained in real time through the LabSolutions workstation, which provides a powerful method for the qualitative analysis of the purity detection of the purification results.
LC-6AD is Shimadzu's infusion pump that can be used for analysis and semi-preparative chromatography. Its infusion flow rate is between 0.01-20.0mL, which can meet the needs of conventional liquid analysis and semi-preparation. This set of preparative liquid chromatography system cooperates with the Shimadzu diode array detector SPD-M20A to collect the target peak in a horizontal manner. At the same time, the peak purity detection function of the PDA data in the LabSolutions workstation can be used to detect the separation result, which is convenient and convenient. Efficient, through the comparison of spectral maps and spectral library search, it can provide a fast and efficient method for the qualitative identification of the fractions obtained after sample preparation and separation.
About Shimadzu
Shimadzu Enterprise Management (China) Co., Ltd. is a local legal person company established in China in 1999 by Shimadzu Corporation to expand the scale of its business in China.
At present, Shimadzu Enterprise Management (China) Co., Ltd. has 13 branches throughout China, and its business scale is continuously expanding. There are analysis centers in Beijing, Shanghai, Guangzhou, Shenyang, and Chengdu; a network of sales agents covering 30 provinces across the country; and more than 60 technical service stations to build a complete system that provides good services to users.
As a global production base, Shimadzu has built a product production and supply system not only for Chinese customers, but also for the world, and strives to build a product production system that meets the requirements of the Chinese market. With the goal of "for the health of mankind and the earth", Shimadzu will always strive to provide users with more advanced products and more satisfactory services.
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