Fault phenomenon:
Peak broadening
possible reason:
(1) The injection volume is too large
(2) Extra-column volume is too large
(3) The mobile phase viscosity is too high
(4) The retention time is too long
(5) Sample overload
(6) The solubility of the buffer is too low
(7) Protection column pollution or failure
(8) Column contamination or failure, column efficiency is too low
(9) presenting two or more peaks that are not completely separated
(10) The column temperature is too low
Method of exclusion:
(1) reduce the injection volume
(2) Check the connections at each joint to avoid dead volume formation
(3) increase the column temperature, using a low viscosity mobile phase
(4) Increase the concentration of strong solvent in isocratic elution, or use gradient elution
(5) Dilute the sample, or use a small volume injection
(6) increase the concentration
(7) Replace the guard column
(8) Cleaning or replacing the column
(9) Select other columns to improve separation
(10) Properly increase the column temperature
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