This kit uses the double antibody sandwich method to determine the level of human neutrophil gelatinase-associated lipocalin (NGAL) in the specimen. Microporous plates were coated with purified human neutrophil gelatinase-associated lipocalin (NGAL) antibody to make solid-phase antibodies, and neutrophil gelatinase-related lipids were added to the microwells coated with monoclonal antibody in turn The carrier protein (NGAL) is combined with HRP-labeled neutrophil gelatinase-related lipocalin (NGAL) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with neutrophil gelatinase-associated lipocalin (NGAL) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human neutrophil gelatinase-related lipocalin (NGAL) in the sample was calculated by a standard curve.
1
8μg / L
Calculation
Taking the concentration of the standard as the abscissa and the OD value as the ordinate, draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or use the concentration of the standard Calculate the linear regression equation of the standard curve with the OD value, substitute the OD value of the sample into the equation, calculate the sample concentration, and multiply it by the dilution factor to obtain the actual concentration of the sample. Precautions
1. The kit should be equilibrated at room temperature for 15-30 minutes before being taken out of the refrigerated environment. If the enzyme label coated plate is unopened, the strip should be stored in a sealed bag.
2. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing.
3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples.
4. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the test substance in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with a certain multiple (n times) of the sample diluent and then determine it. Multiple (× n × 5).
5. The sealing film is limited to one-time use to avoid cross-contamination.
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined by the microplate reader.
8. All samples, washing liquids and various wastes should be treated as infectious agents.
9. The components of different batches of this reagent shall not be mixed.
10. If there is any difference with the English manual, the English manual shall prevail.
Storage conditions and validity period
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months
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The appeal was the friendly shape of the blade: 5 to 7 inches long, with a rounded front edge and a boxier build than the typical chef`s knife, which usually stretches between 8 and 10 inches long and has a sleeker profile and a sword-like point. The style was developed for postwar Japanese home cooks as a more versatile alternative to vegetable cleavers- santoku reportedly means [three virtues," which are described variously as [meat, fish, and vegetables," or [chopping, slicing, and dicing"-and quickly became the country`s most popular Kitchen Knife.
Santoku Knife, 7-Inch Santoku Knife, Japanese Knife
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