Enzyme-linked immunosorbent assay for quantitative determination of melamine residues. The melamine in the sample was extracted by the extraction solution through homogenization and shaking for immunoassay. First add the melamine enzyme marker, sample extract and standard to the microwells that have been coated with melamine antibody to start the reaction. During the 30-minute incubation process, the melamine in the sample extract competes with the melamine enzyme label to bind the melamine antibody in the microwell. After 30 minutes of incubation, all unbound melamine and melamine enzyme labels in the well are washed away. After washing with deionized water, a clear substrate solution is added to each well, and the bound enzyme label converts the colorless substrate into a blue substance. After incubating for 30 minutes, the reaction was stopped, and the data was read according to the color of each well. According to the standard color, the concentration value of melamine in the sample is obtained.
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