This reagent is for research use only: This kit is used to determine the content of glutathione peroxidase (GSH-PX) in rat serum, plasma, cell supernatant and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of rat glutathione peroxidase (GSH-PX) in the specimen. The microplate was coated with purified rat glutathione peroxidase (GSH-PX) antibody to prepare a solid phase antibody, and glutathione peroxidase was sequentially added to the microcapsule of the coated monoclonal antibody. GSH-PX) is then combined with HRP-labeled glutathione peroxidase (GSH-PX) antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with glutathione peroxidase (GSH-PX) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat glutathione peroxidase (GSH-PX) in the sample was calculated from a standard curve. Kit composition: Kit composition 48-well configuration 96-well configuration Storage instructions 1 part 1 part sealing film 2 pieces (48) 2 pieces (96) Sealed bag 1 1 enzyme label coated plate 1 × 48 1 × 96 2 -8 ° C preservation standard: 45 pmol / ml 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ° C preservation standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ° C preservation enzyme standard reagent 3 ml ×1 bottle 6 ml×1 bottle 2-8°C Preservation sample dilution 3 ml×1 bottle 6 ml×1 bottle 2-8°C Preservation developer A solution 3 ml×1 bottle 6 ml×1 bottle 2-8°C Preservation developer B solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation solution 3 ml × 1 bottle 6 ml × 1 bottle 2-8 ° C Preservation concentrated washing solution (20ml × 20 times) × 1 bottle (20ml ×30 times) × 1 bottle 2-8 ° C preservation
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