The principle of detecting relevant antigens in tissues or organs with enzyme-labeled test paper is as described above. The conjugate of enzyme and protein (such as enzyme-labeled antibody) is relatively stable within a certain period of time. Especially the combination of horseradish peroxidase, after freeze-drying into a powder, the effective time at room temperature storage is one and a half years; the effective time at -20 ℃ is about 3 years. When the enzyme-labeled antibody solution penetrates into the cellulose acetate film, it remains in the loose structure of the film. After natural drying, the enzyme-labeled antibody will not lose activity for at least one and a half years. Cellulose acetate membranes containing enzyme-labeled antibodies are called Enzyme-Linked Test Paper (ELTP). Treat tissues or organs in a certain way, such as plant leaves, fix intracellular antigens, remove waxy layers and pigments, and fully inhibit endogenous enzymes, then wash them with PBS, apply enzyme-labeled antibody test paper to the wet leaves On the surface, after incubation for a certain period of time, the enzyme-labeled antibody in the enzyme-labeled antibody test paper will bind to the relevant viral antigen in the leaf, and then use the substrate to develop color to achieve the detection purpose. Using enzyme-labeled test paper to detect the relevant viral antigens in tissues or organs (such as the entire leaf) is simple and convenient, and can keep the leaves unchanged.
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